European Science Methodical Journal

DETECTED TOXOPLASMA GONDII IN WOMEN AND STUDY ITS ROLE IN INHIBITING CYTOCHROME C-INDUCED CASPASE ACTIVATION IN ITS HOST CELL

Russul Wassit Kadhum

Department of Biology, College of Sciences, University of Wasit, Iraq

Keywords: immunohistochemistry, Toxoplasma gondii, inhibiting cytochrome c-induced.

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Abstract

The purpose of the current study is detecting the role in inhibiting cytochrome c-induced caspase activation in its host cell. 115 samples of placenta aborted women were collected from women whom attended to (Al –kut Hospital for Gyneclogy and obstetrics and pediatrics) from 15 October 2022 to 15 June 2023. The study showed the prevalence of Toxoplasma gondii among abortion women in Waist province was 32 (27.9%). DNA was extracted from the positive samples, then the PCR-RFLP method was applied in the GRA6 gene the examination showed three types of Toxoplasma, Type I was the most prevalent, and Type III was the least prevalent. The percentages were ( 59.3% , 37.5% and 3.1% for the Type I ,Type II , and Type III, respectively. immunohistochemistry technique used to to detect the intensity of expression of caspases 8 and caspases 3, and the study showed weak expression of caspases. 8 While the expression intensity of caspase-3 differed between low, medium and high and according to the difference of the Type, the study concludes the programmed cell death was by intrinsic effect in samples of the placenta of aborted women by using the immunohistochemistry method.