Analysis Of Bioactive Compounds in Ethanolic Extract of Xylopia Aethiopica Leaves Using Gas Chromatography and Mass Spectrometry (GC-MS) Technique
Alagbe, J.O., Kadiri, Mercy. C.
Department of Animal Nutrition and Biochemistry, Sumitra Research Institute, Gujarat, India
Oluwafemi, R.A., Agubosi, O.C.P, Anorue, D. N.
Department of Animal Science, University of Abuja, Nigeria
Keywords:
Xylopia aethiopica, anti-microbial, phytochemicals, gas chromatography, mass spectrometry, free radicals.
Abstract
This study investigated the bioactive compounds in ethanolic extract of Xylopia aethiopica leaves using gas chromatography and mass spectrometry (GC-MS) technique. The phytoconstituents present in Xylopia aethiopica leaves were: flavonoids (951.82 mg/g), tannins (282.70 mg/g), alkaloids (188.47 mg/g), phenols (603.25 mg/g), saponins (11.47 mg/g), steroids (91.20 mg/g), oxalates (190.32 mg/g) and glycosides (190.32 mg/g). A total number of 30 bioactive compounds were identified based on their peak areas. The major compounds greater than 1 % were; 13-docosenamide (21.09 %), terpineol (10.07 %), 1,6-cyclodecadiene (9.37 %), copaene (2.88 %), caryophyllene (8.15 %), β-ocimene (6.05 %), β-myrcene (5.09 %), copaene (2.38 %), 2 – methoxy-4-vinylphenol (1.72 %), β-elemenone (1.31 %), 3,4-dimethylphenyl heptyl ether (1.26 %), ethyl oleate (1.07 %) and γ-elemene (1.27 %) while those less than 1 % (˂ 1 %) were; 2- methylenebornane (0.66 %), 2-methoxy-2-prophenyl (0.72 %), hexadec-7-enal (0.23 %), hexadecanoic acid (0.02 %), didodecyl benzene 1,2 dicarboxylate (0.09 %), methyl stearate (0.16 %), 9,12-octadecadienoic acid (0.08 %), hexadeca-7,10 – dienal (0.47 %), 1,1,5 –trimethyl -1,2-dihydronaphthalene (0.01 %), propane, 1,1 – oxybis -3- chloro (0.08 %), 1-trimethylsilypent-1-en-4-yne (0.03 %), bicyclo[13.1.0] hexade can-2-one (0.02 %), methyl octadeca-9-yn-11-trans-enoate (0.51 %), cis-linaloxide (0.22 %), tetradecanoic acid, 10,13 –dimethyl ester (0.18 %), didodecyl benzene 1,2 –dicarboxylate (0.47 %) and 2-cyclopentene -1-one, 2 – hyroxy (0.09 %). However, all the compounds have a wide range of pharmacological activities including- antimicrobial, antioxidant, anti-malarial, antifungal, anti-arrhythmic, anti-viral, hepato-protective, anti-proliferative, anti-depressant, antipyretic and antihelminthic.
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